The painting depicts the biblical episode of the Sacrifice of Noah (Genesis 8:20-22), which took place after the end of the Great Flood as thanksgiving to God.
In the center: The senile figure of Noah with his arms wide open in prayer towards heaven.
Left: A sacrificial altar from which flames and smoke emanate; above, among the clouds, appears the divine figure blessing the offering, accompanied by a small putto.
Right and foreground: Noah's family members in various devotional poses, a young man leading animals, and, lower right, "still life" pieces with pottery and a dog.
In the background: A rugged landscape with a high mountain (probably Ararat) under a sky that hints at the thinning of post-diluvian clouds.